Improved explanation for how to change sequence methylation when an enzyme is blocked during Restriction Cloning.Added support for pasting copied complementary primer pairs to configure an Agarose Gel lane.Features and custom numbering are now retained when copying and pasting into the New Protein File dialog, or inserting or replacing residues in a protein sequence.Added Gateway Cloning destination vectors pEXP3-DEST and pEXP4-DEST, and updated cross-references in descriptions for pEXP-DEST Vectors.Added support for including binding site locations when exporting primer data.Convert Psi to U when importing or creating a new RNA sequence.Note that the locking mechanism is not available on large files, and has short delay, so is not effective when files are opened simultaneously Added a locking mechanism which sets files as read only to other instances of SnapGene when being edited.Added Tm for the selected region in ssDNA sequences.Switched from "?" to "X/Xaa" in translations to represent ambiguous amino acids.Support for optionally displaying sequence names alongside the sequences when printing pairwise and multiple sequence alignments.Enhanced the 'Blocks of 3' Sequence view format to enable aligning nucleotide triplets with the reading frame.Added base counts to the DNA Calculations dialog.Added tool for converting ng/uL to nM in the DNA Calculations dialog.Enabled setting a default Codon Usage Table.Included controls to filter the set of chosen enzymes based on the number of times and relative location an enzyme cleaves with respect to its recognition sequence.Enhanced cloning simulation tools to support adding, removing and re-ordering sequences.Enhanced Gibson, InFusion and NEBuilder HiFi Assembly simulation tools to allow the vector to be flipped.Enabled searching an alignment to find features.Added a features table when viewing pairwise and multiple sequence alignments.Enabled adding, editing and deleting features in alignments.Added support for features within pairwise alignments.Added support for saving and loading Agarose gel simulations as.Enabled changing the default color of standard Genbank feature types.Added support for custom features types not included in the standard set of GenBank feature types.Added a tool for removing an enzyme site from a coding sequence by silent mutation.Added a tool for adding enzymes sites to a coding sequence by silent mutation.
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